Application

Immunohistochemistry: 1:2,000-1:3,000. The antibody reacts well with tissue from the rat spinal cord.
Immunoblotting: 1:1,000-1:1,500 on rat membrane preparations. The antibody should be
evaluated for use in Western blot by the end user. Its utility will be dependant on the level of
the protein expressed in the tissues or cells being tested.
Optimal working dilutions must be determined by end user.

Research CategoryNeuroscience

Research Sub CategoryNeurochemistry & Neurotrophins

Anti-Trk B Antibody, pain, a.a. 54-67 rTrkB detects level of Trk B & has been published & validated for use in IH & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: a.a. 54-67 rtrkB

A synthetic peptide from rat trkB

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Rabbit serum. Lyophilized. Contains no preservative. Reconstitute with 50 µL of sterile distilled water. Centrifuge if necessary to remove any residue.

Specificity

TrkB. The sequence used for the immunogen was chosen to avoid cross reactivity with trkA or trkC.

Storage and Stability

Maintain lyophilized material at -20°C to -70°C for up to 6 months after date of receipt. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.